Review



sv40 nuclear localization sequence  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    Addgene inc sv40 nuclear localization sequence
    Sv40 Nuclear Localization Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nuclear+localization+sequence+nls/pmCherry-NLS+(Plasmid+%2339319)/pm39779212-159-1-6
    Average 93 stars, based on 25 article reviews
    sv40 nuclear localization sequence - by Bioz Stars, 2026-09
    93/100 stars

    Images

    Related Articles

    Sequencing:

    Article Title: Dynamics of Notch-Dependent Transcriptional Bursting in its Native Context
    Article Snippet: This construct contains MS2 coat protein tagged with a codon optimized superfolder GFP (MCP::GFP) and mCherry-tagged histone 2B (H2B::mCherry), driven by the mex-5 promoter and tbb-2 (for MCP::GFP) and unc-54 (for H2B::mCherry) 3’end regulatory sequences ( ); MS2 coat protein (Addgene #27121) was fused with superfolder GFP ( ); Histone 2B was fused with mCherry ( ); gpd-2 intergenic sequence was used to generate a trans-spliced operon ( ). .. Of note, the nuclear localization sequence (NLS) in the original construct (Addgene #27121) ( ) was removed to minimize the MCP::GFP background in the nucleus. ..

    Article Title: Ribosome-Associated Vesicles promote activity-dependent local translation
    Article Snippet: 4xmts-mNeonGreen (Addgene #98876) consists of mNeonGreen fused to the mitochondria-targeted COX8 sequence 3′ to the fluorophore (2). .. NLS-stdMCP-stdGFP consists of a fusion of synonymous versions of MCP (stdMCP) and GFP (stdGFP) with a nuclear localization sequence (NLS) (Addgene #98916). .. A fluorescent SunTag reporter of mRNA translation was modified from the original SINAPS construct (pUbC-FLAG-24xSuntagV4oxEBFP-AID-baUTR1-24xMS2V5-Wpre; Addgene #84561)(3).

    Article Title: MicroID2: A Novel Biotin Ligase Enables Rapid Proximity-Dependent Proteomics
    Article Snippet: MicroID2 and TurboID constructs were cloned into the sleeping beauty backbone pSBbi-RB (Addgene; plasmid no.: 60522). .. Site-directed mutagenesis or restriction enzyme cloning was utilized to introduce localization sites: 3× nuclear localization sequence (NLS) (Addgene; plasmid no.: 98875), mitochondrial targeting sequence (MTS) (Addgene; plasmid no.: 98876), and the nuclear export sequence (NES) (Addgene; plasmid no.: 49386). .. The final constructs were transfected into BEAS-2B and cotransfected with the sleeping beauty transposase (pCMV(CAT)T7-SB100; Addgene; plasmid no.: 34879) at a 1:5 ratio.

    Article Title: Dynamics of Notch-Dependent Transcriptional Bursting in Its Native Context
    Article Snippet: This construct contains MS2 coat protein tagged with a codon optimized superfolder GFP (MCP::GFP) and mCherry-tagged histone 2B (H2B::mCherry), driven by the mex-5 promoter and tbb-2 (for MCP::GFP) and unc-54 (for H2B::mCherry) 3’end regulatory sequences ( Merritt et al., 2008 ); MS2 coat protein (Addgene #27121) was fused with superfolder GFP ( Kersey et al., 2016 ); Histone 2B was fused with mCherry ( Merritt et al., 2008 ); gpd-2 inter-genic sequence was used to generate a trans-spliced operon ( Huang et al., 2001 ). .. Of note, the nuclear localization sequence (NLS) in the original construct (Addgene #27121) ( Fusco et al., 2003 ) was removed to minimize the MCP::GFP background in the nucleus. ..

    Article Title: Evolutionarily conserved TRH neuropeptide pathway regulates growth in Caenorhabditis elegans
    Article Snippet: Briefly, the targeting oligonucleotides ( ) were inserted into the EcoRI site between the rpr-1 promoter and the gRNA scaffold (Addgene plasmid no. 48961) by Gibson assembly. .. C . elegans codon-optimized Streptococcus pyogenes Cas9 nuclease with a nuclear localization sequence (NLS) and 3×FLAG tag (Addgene plasmid no. 48960) was used as the Cas9 expression plasmid. ..

    Article Title: A CRISPR/Cas13-based approach demonstrates biological relevance of vlinc class of long non-coding RNAs in anticancer drug response
    Article Snippet: .. The pC034-LwCas13a-msfGFP-2A-Blast plasmid expressing LwCas13a protein stabilized by fusion with msfGFP (LwCas13a-msfGFP) and flanked by N-terminal and C-terminal nuclear localization sequence (NLS) was a gift from Feng Zhang (Addgene plasmid #91924; http://n2t.net/addgene:91924 ; RRID: Addgene_91924). ..

    Construct:

    Article Title: Dynamics of Notch-Dependent Transcriptional Bursting in its Native Context
    Article Snippet: This construct contains MS2 coat protein tagged with a codon optimized superfolder GFP (MCP::GFP) and mCherry-tagged histone 2B (H2B::mCherry), driven by the mex-5 promoter and tbb-2 (for MCP::GFP) and unc-54 (for H2B::mCherry) 3’end regulatory sequences ( ); MS2 coat protein (Addgene #27121) was fused with superfolder GFP ( ); Histone 2B was fused with mCherry ( ); gpd-2 intergenic sequence was used to generate a trans-spliced operon ( ). .. Of note, the nuclear localization sequence (NLS) in the original construct (Addgene #27121) ( ) was removed to minimize the MCP::GFP background in the nucleus. ..

    Article Title: Dynamics of Notch-Dependent Transcriptional Bursting in Its Native Context
    Article Snippet: This construct contains MS2 coat protein tagged with a codon optimized superfolder GFP (MCP::GFP) and mCherry-tagged histone 2B (H2B::mCherry), driven by the mex-5 promoter and tbb-2 (for MCP::GFP) and unc-54 (for H2B::mCherry) 3’end regulatory sequences ( Merritt et al., 2008 ); MS2 coat protein (Addgene #27121) was fused with superfolder GFP ( Kersey et al., 2016 ); Histone 2B was fused with mCherry ( Merritt et al., 2008 ); gpd-2 inter-genic sequence was used to generate a trans-spliced operon ( Huang et al., 2001 ). .. Of note, the nuclear localization sequence (NLS) in the original construct (Addgene #27121) ( Fusco et al., 2003 ) was removed to minimize the MCP::GFP background in the nucleus. ..

    Mutagenesis:

    Article Title: MicroID2: A Novel Biotin Ligase Enables Rapid Proximity-Dependent Proteomics
    Article Snippet: MicroID2 and TurboID constructs were cloned into the sleeping beauty backbone pSBbi-RB (Addgene; plasmid no.: 60522). .. Site-directed mutagenesis or restriction enzyme cloning was utilized to introduce localization sites: 3× nuclear localization sequence (NLS) (Addgene; plasmid no.: 98875), mitochondrial targeting sequence (MTS) (Addgene; plasmid no.: 98876), and the nuclear export sequence (NES) (Addgene; plasmid no.: 49386). .. The final constructs were transfected into BEAS-2B and cotransfected with the sleeping beauty transposase (pCMV(CAT)T7-SB100; Addgene; plasmid no.: 34879) at a 1:5 ratio.

    Cloning:

    Article Title: MicroID2: A Novel Biotin Ligase Enables Rapid Proximity-Dependent Proteomics
    Article Snippet: MicroID2 and TurboID constructs were cloned into the sleeping beauty backbone pSBbi-RB (Addgene; plasmid no.: 60522). .. Site-directed mutagenesis or restriction enzyme cloning was utilized to introduce localization sites: 3× nuclear localization sequence (NLS) (Addgene; plasmid no.: 98875), mitochondrial targeting sequence (MTS) (Addgene; plasmid no.: 98876), and the nuclear export sequence (NES) (Addgene; plasmid no.: 49386). .. The final constructs were transfected into BEAS-2B and cotransfected with the sleeping beauty transposase (pCMV(CAT)T7-SB100; Addgene; plasmid no.: 34879) at a 1:5 ratio.

    Introduce:

    Article Title: MicroID2: A Novel Biotin Ligase Enables Rapid Proximity-Dependent Proteomics
    Article Snippet: MicroID2 and TurboID constructs were cloned into the sleeping beauty backbone pSBbi-RB (Addgene; plasmid no.: 60522). .. Site-directed mutagenesis or restriction enzyme cloning was utilized to introduce localization sites: 3× nuclear localization sequence (NLS) (Addgene; plasmid no.: 98875), mitochondrial targeting sequence (MTS) (Addgene; plasmid no.: 98876), and the nuclear export sequence (NES) (Addgene; plasmid no.: 49386). .. The final constructs were transfected into BEAS-2B and cotransfected with the sleeping beauty transposase (pCMV(CAT)T7-SB100; Addgene; plasmid no.: 34879) at a 1:5 ratio.

    Plasmid Preparation:

    Article Title: MicroID2: A Novel Biotin Ligase Enables Rapid Proximity-Dependent Proteomics
    Article Snippet: MicroID2 and TurboID constructs were cloned into the sleeping beauty backbone pSBbi-RB (Addgene; plasmid no.: 60522). .. Site-directed mutagenesis or restriction enzyme cloning was utilized to introduce localization sites: 3× nuclear localization sequence (NLS) (Addgene; plasmid no.: 98875), mitochondrial targeting sequence (MTS) (Addgene; plasmid no.: 98876), and the nuclear export sequence (NES) (Addgene; plasmid no.: 49386). .. The final constructs were transfected into BEAS-2B and cotransfected with the sleeping beauty transposase (pCMV(CAT)T7-SB100; Addgene; plasmid no.: 34879) at a 1:5 ratio.

    Article Title: Evolutionarily conserved TRH neuropeptide pathway regulates growth in Caenorhabditis elegans
    Article Snippet: Briefly, the targeting oligonucleotides ( ) were inserted into the EcoRI site between the rpr-1 promoter and the gRNA scaffold (Addgene plasmid no. 48961) by Gibson assembly. .. C . elegans codon-optimized Streptococcus pyogenes Cas9 nuclease with a nuclear localization sequence (NLS) and 3×FLAG tag (Addgene plasmid no. 48960) was used as the Cas9 expression plasmid. ..

    Article Title: A CRISPR/Cas13-based approach demonstrates biological relevance of vlinc class of long non-coding RNAs in anticancer drug response
    Article Snippet: .. The pC034-LwCas13a-msfGFP-2A-Blast plasmid expressing LwCas13a protein stabilized by fusion with msfGFP (LwCas13a-msfGFP) and flanked by N-terminal and C-terminal nuclear localization sequence (NLS) was a gift from Feng Zhang (Addgene plasmid #91924; http://n2t.net/addgene:91924 ; RRID: Addgene_91924). ..

    Expressing:

    Article Title: Evolutionarily conserved TRH neuropeptide pathway regulates growth in Caenorhabditis elegans
    Article Snippet: Briefly, the targeting oligonucleotides ( ) were inserted into the EcoRI site between the rpr-1 promoter and the gRNA scaffold (Addgene plasmid no. 48961) by Gibson assembly. .. C . elegans codon-optimized Streptococcus pyogenes Cas9 nuclease with a nuclear localization sequence (NLS) and 3×FLAG tag (Addgene plasmid no. 48960) was used as the Cas9 expression plasmid. ..

    Article Title: A CRISPR/Cas13-based approach demonstrates biological relevance of vlinc class of long non-coding RNAs in anticancer drug response
    Article Snippet: .. The pC034-LwCas13a-msfGFP-2A-Blast plasmid expressing LwCas13a protein stabilized by fusion with msfGFP (LwCas13a-msfGFP) and flanked by N-terminal and C-terminal nuclear localization sequence (NLS) was a gift from Feng Zhang (Addgene plasmid #91924; http://n2t.net/addgene:91924 ; RRID: Addgene_91924). ..



    Similar Products

    93
    Addgene inc sv40 nuclear localization sequence
    Sv40 Nuclear Localization Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nuclear+localization+sequence+nls/pmCherry-NLS+(Plasmid+%2339319)/pm39779212-159-1-6
    Average 93 stars, based on 1 article reviews
    sv40 nuclear localization sequence - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    90
    GenScript corporation cpf1 fused to the sv40 nuclear localization sequence (nls)
    Cpf1 Fused To The Sv40 Nuclear Localization Sequence (Nls), supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nuclear+localization+sequence+nls/cpf1+proteins+codon+optimized+human+expression/pm39838422-62-18-31
    Average 90 stars, based on 1 article reviews
    cpf1 fused to the sv40 nuclear localization sequence (nls) - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Addgene inc linearized dna fragment mcherry marker fused with a nuclear localization sequence (nls)
    Linearized Dna Fragment Mcherry Marker Fused With A Nuclear Localization Sequence (Nls), supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nuclear+localization+sequence+nls/linearized+dna+fragment+mcherry+marker+fused+with+a+nuclear+localization+sequence++nls+/pm38995464-70-3-20
    Average 90 stars, based on 1 article reviews
    linearized dna fragment mcherry marker fused with a nuclear localization sequence (nls) - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    94
    Addgene inc nuclear localization sequence nls
    Nuclear Localization Sequence Nls, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nuclear+localization+sequence+nls/pUbC-nls-ha-stdMCP-stdGFP+(Plasmid+%2398916)/pmc11185778__media___1-10-16-20
    Average 94 stars, based on 1 article reviews
    nuclear localization sequence nls - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    90
    Synpeptide Co Ltd nuclear localization signal peptide (sequence: pkkkrkv, nls)
    Nuclear Localization Signal Peptide (Sequence: Pkkkrkv, Nls), supplied by Synpeptide Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nuclear+localization+sequence+nls/nuclear+localization+signal+peptide++sequence++pkkkrkv++nls+/pm37975509-266-1-11
    Average 90 stars, based on 1 article reviews
    nuclear localization signal peptide (sequence: pkkkrkv, nls) - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    TriLink cas9-encoding mrna comprising nuclear localization sequence (cas9-nls mrna
    Cas9 Encoding Mrna Comprising Nuclear Localization Sequence (Cas9 Nls Mrna, supplied by TriLink, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nuclear+localization+sequence+nls/cas9+mrna/us11608500-1631-4-16
    Average 90 stars, based on 1 article reviews
    cas9-encoding mrna comprising nuclear localization sequence (cas9-nls mrna - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    GenScript corporation flag epitope and nuclear localization signal (nls) fusions codon-optimized cas9 coding sequence
    Flag Epitope And Nuclear Localization Signal (Nls) Fusions Codon Optimized Cas9 Coding Sequence, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nuclear+localization+sequence+nls/cas9+protein/pm36751129-234-1-19
    Average 90 stars, based on 1 article reviews
    flag epitope and nuclear localization signal (nls) fusions codon-optimized cas9 coding sequence - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Addgene inc human codon optimized s. pyogenes cas9 with both n-terminal and c-terminal nuclear localization sequences (nls-cas9-nls)
    Human Codon Optimized S. Pyogenes Cas9 With Both N Terminal And C Terminal Nuclear Localization Sequences (Nls Cas9 Nls), supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nuclear+localization+sequence+nls/px330+u6+chimeric+bb+cbh+hspcas9/us11555207-395-14-24
    Average 90 stars, based on 1 article reviews
    human codon optimized s. pyogenes cas9 with both n-terminal and c-terminal nuclear localization sequences (nls-cas9-nls) - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    Image Search Results